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摘要:
本发明公开了一种观察外植体发生途径的方法,即利用燕子掌的叶片和茎段作为外植体接种到含不同浓度的植物生长调节剂的MS培养基中,30天后能观察到无菌短枝型、丛生芽增殖型、胚状体发生型、器官发生型这四种不同的外植体发生途径,本发明方法具有取材方便、操作简单、观察效果好的特点。优化了教学过程,提高了教学效果;有利于学生实践能力和观察能力的培养。包括以下步骤:(1)材料的获得;(2)无菌处理;(3)无菌短枝型;(4)丛生芽增殖型;(5)胚状体发生型;(6)器官发生型Ⅰ;(7)器官发生型Ⅱ。
【翻译】The present invention discloses a method for observing explant generation pathway. inoculating the leaf and stem of swallow palm as explant into MS culture medium containing plant growth regulator in different concentration, After 30 days, four different explant generation paths of aseptic short branch type, cluster bud proliferation type, embryogenic type and organogenesis type can be observed. The method has the characteristics of convenience in material obtaining, simplicity in operation and good observation effect. Optimizing the teaching process and improving the teaching effect; The utility model is favorable for cultivating the practical ability and the observing ability of students. Includes the following steps : (1) obtaining material; (2) aseptic treatment; (3) aseptic short branch type; (4) multiplication type of cluster buds; (5) embryogenic form; (6) organogenesis type I; (7) organogenesis type II.
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